Histochemical and immunohistochemical investigation of pelvic symhysis of Holstein-Friesian cattle
Although the knowledge on morphological changes in cattle pelvic symphysis during gestation and obstetrics gives valuable information about the age of optimal primary calving of cows, up to now little research has been carried out about the ossification of pelvic symphysis. The aim of the study was to carry out histochemical and immunohistochemical comparative investigation of pelvic symphysis in calves, in-calfed and after-calved Estonian Holstein- Friesian’s (EHF) cattle.
The material from the cranial part of the pubic bone, pubo-ischiadic junction, symphyseal eminence and the body of the interischiadic bone was taken for immunohistochemical investigation from eight EHF cows divided into three age groups: calves, in-calfed and after-calved EHF’s. Specimen were fixed with 10% formalin, demineralized thereafter in EDTA and embedded into paraffin, slices 7 μm in thickness were cut followed by histochemical staining by Safranin-O and immunohistochemical staining by primary antibodies Collagen-II and Periostin (Novus Biologicals, Littleton, CO, USA). Immunohistochemical staining was carried out according to the manufacturers quidleines (Invitrogen, Carlsbad, CA, USA).
Our investigations proved that the main morphological changes, the retardation of ossification processes, in the pelvic symphysis occur in adult EHF kines during the second-half of gestation in the pubo-ischiadic junction.