Mouse Lung Conditioned Medium Induces Short Term Erythropoiesis in Mouse Long Term Bone Marrow Culture System

Abstract

Dexter-type long-term bone marrow culture is a myelopoietic culture system that allows maintenance of  mouse and human hematopoiesis in vitro over a period of several months. In mouse unperturbed long-term  bone marrow culture, erythropoiesis activity is limited to the production of immature erythroid progenitors  (BFU-E) from primitive hematopoietic stem cells. In this study the effects of mouse lung conditioned  medium (MLCM) as a source of myeloid growth factors, on long-term mouse bone marrow cultures was  studied. Numbers of cells in adherent and non-adherent layers of cultures were counted weekly and the  morphological appearances of mature cells that were produced in non-adherent layers were analyzed. In the  presence of MLCM, mature nonnucleated and hemoglobinized red blood cells were produced in the nonadherent  layers of the cultures.