Case Study: Mouse Parvovirus Outbreak Likely Caused by a Contaminated Commercial Lyophilized Antibody Powder

Abstract

An MPV-contaminated lyophilized antibody product obtained from a commercial vendor was the probable cause of an outbreak of mouse parvovirus (MPV) in an academic research institution. The outbreak was initially discovered by the seroconversion of the mouse sentinels receiving soiled bedding from the affected cage(s). After further investigation, a suspected antibody product was submitted to a diagnostic laboratory and the sample tested positive for MPV via polymerase chain reaction (PCR). To confirm administration of this product to mice could produce MPV infection, we inoculated the MPV-positive antibody product into experimental mice (n=5). We collected faecal pellets at Days 0, 5, 9, 12, and 14 post-inoculation. At the end of the experimental period, we collected mesenteric lymph nodes (mLN) and submitted both mLN and faecal pellets for MPV analysis via PCR. While all faecal pellets were negative for MPV, we were able to detect MPV in mLN from one of the five mice, thus replicating the likely method of transmission and the cause of the MPV outbreaks.