Strain restricted typing sera for the use in the genetic monitoring of inbred strains of mice and rats from two danish SPF breeders

Abstract

Strain restricted typing sera (SRTS) were produced for some of the most used inbred mouse and rat strains in Denmark by injecting lymphocytes pooled from several different strains intraperitoneally into the recipients. The SRTS were combined with a complement dependent cytotoxieity assay. It was possible to distinguish animals from strains with different MHC haplotypes but not animals from strains with the same MHC haplotype. It was concluded that the SRTS eomhined with a  complement dependent cytotoxicity assay could distinguish between some of the most commonly used inbred strains of rats and mice in Denmark.