The isolated perfused mouse uterus as a model for the study of implantation in vitro. Methodology and morphology

Abstract

In order to facilitate investigations of mammalian blastocyst implantation in the endometrium, an in-vitro organ perfusion technique was developed. This technique was designed to avoid the drawbacks of inVivo and cell culture investigations, while retaining physiological resolution of the endo- and paracrinology and specifically a normal epithelium to stroma relationship. The ovary, oviduct and uterine horn from 21 mice were perfused in-vitro for 10 hours. The surgical techniques for isolation of the organs as well as the perfusion procedure are described. The resultant morphology of the perfused tissue, including implantations is described and illustrated by light and transmission electron microscopy. The model seems to be useful for studying the mammalian implantation as implantation takes place and decidua is formed during perfusion.