Bacteriological quality and intake of acidified drinking water in Wistar rats is pH-dependent
The effects of acidification of drinking water on bacteriological quality and water intake in adult, male Wistar rats. was studied in 2 consecutive experiments. HCl was used to aeidify water to pH 2.5, 3.0 and 3.5. Control groups received untreated
tap or demineralized water. Acidification of water with HCl to pH 2.5 effectively prevented growth of aerobic bacteria in the drinking water bottles after a number of days, but also caused a reproducible decline in water intake when compared to untreated water. A reduced water intake may indicate disturbed wellbeing and may interfere with experimental results. Acidification to pH 3.0 also kept bacteriological counts low and did not reduce water intake when compared to rats drinking
non-acidified water. Acidification to pH 3.5 led to high bacteriological counts after a few days. On the basis of these 2 experiments, acidification of drinking water with 1101 to pH 3.0 is advised.