Possible role ofantibodies to leptin as growth promoters — preliminary study in mice

Abstract

The aim was to investigate the effect on normal mice of an intraperitoneal injection of antibodies to leptin. Eight weaned male mice (30-day-old, average weight 33 g), were divided into two groups (n = 4). C (Control) and T (Treated), and fed ad libtium standard milled mice chow. Treated animals were injected i.p. twice a day (12 h interval) with a 0.2 ml solution of Anti-mouse Leptin antibody (4 pg / 0.2 ml of sterile placebo); control animals were also injected twice a day with 0.2 ml of sterile placebo. At day 10 the mice were killed, carcasses dressed and frozen, and their characteristics determined. No significant differences were recorded possibly suggesting lack efeffect of the leptin antibody.