A rat model for the immune response to the intrauterine administration of BCG

Abstract

This study was designed to investigate the changes in the numbers of lymphocytes, macrophages and plasma cells in the uterus and ileocecal lymph nodes of rats exposed to the intrauterine administration of Baeillus-Calmette Guerin (BCG). Thirty female Wistar Albino rats, age 6 months and weighing between 200-250 g, were assigned to the two experimental groups BCG treated and controls (n = 15). The intrauterine BCG injections were made using laparatomy in the diestrous cycle under Rompun and Ketalar anesthesia. 0.1 ml BCG were injected for each into cornu uteri while the control group received 0.1 ml sterile saline in the same place. Two weeks later, the rats in both groups were anesthetized with ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha naphthyl acid esterase (ANAE) - positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method; It was found that the numbers of lymphocytes macrophages and plasma cells on the uterus increased (P<0.01) in BCG treated rats. In addition, the number of these cells also increased in the ileoeeeal lymph nodes indicating the presence of an immune response to the intrauterine BCG administration, It is concluded that although the rat was chosen as a model and ECG was given by the process of laparatomy in this study, intracervical administration of BCG tn the uterus should be studied clinically in cases of immune deficiency disorders related to the uterus. such as endometritis, myometritis, pyometra, endometriosis. infertility and implantation problems of domestic animals, to see if there is an increase in the immune response.